Reconstitution
How to Reconstitute Peptides: Units, Math, Step-by-Step
Every peptide vial ships as a freeze-dried powder. Before the first dose you mix it with bacteriostatic water, a process called reconstitution. The math is simple, but the wrong unit (mL versus units on the syringe) is the most common source of 10x overdoses in self-administered protocols.
The short answer
To reconstitute a peptide, wipe both rubber stoppers with alcohol, then inject bacteriostatic water slowly down the inner wall of the freeze-dried vial and swirl gently until the liquid runs clear, never shake it. Then work in insulin-syringe units, not mL: the calculator on this page turns your vial size, bac water volume and target amount into a unit count, so there is no decimal place to misread.
Why units, not mL
Published research figures are reported on this site in insulin-syringe units, never in mL. Reading a decimal place wrong on a small syringe barrel can mean a 10x overdose. Reading the wrong tick mark cannot. The only mL number on this page is the bac water input volume.
The 4-step method
Total time: about 5 minutes. Reusable across every peptide in the Peptara catalog.
Wipe vial top with alcohol
Wipe the rubber stopper of both the peptide vial and the bacteriostatic water vial with a fresh alcohol swab. Let each surface air-dry for a few seconds. This is the single biggest contamination guard in the whole process.
Draw bac water with syringe
Pull the plunger of a U-100 insulin syringe to the bac water volume you have planned for this vial (the recommended volume table below has presets for every Peptara SKU). Insert the needle into the bac water vial and draw cleanly.
Inject slowly down the side of the vial wall
Push the needle into the peptide vial at an angle so the bac water runs down the inner glass wall, not directly onto the lyophilized powder. A slow stream protects fragile peptide bonds and keeps the solution clear.
Swirl gently, do not shake
Roll the vial between your palms or swirl it on a flat surface until the powder is fully dissolved. Shaking creates foam and can fragment the peptide. The solution should look clear within 30 to 60 seconds.
Reconstitution math (units, not mL)
One universal formula handles every peptide in the catalog.
The formula
units = (desired dose in mg / vial strength in mg) × (bac water in mL × 100)
A U-100 insulin syringe holds 100 units per 1mL. So 1mL of reconstituted solution equals 100 units. If you put 10mg of peptide into 1mL of bac water, each unit delivers 0.1mg (100mcg).
Worked examples
Retatrutide
20mg vial, 2mL bac water, 5mg target dose. (5 / 20) × (2 × 100) = 50 units per injection.
Tirzepatide
20mg vial, 2mL bac water, 5mg target dose. Same math as Retatrutide: 50 units per injection.
BPC-157
20mg vial, 2mL bac water, 250mcg (0.25mg) target dose. (0.25 / 20) × 200 = 2.5 units, round up to 3 units per injection.
Calculator
Pick a vial from the Peptara catalog (or enter a custom strength), set your bac water volume, and enter your desired dose. The calculator returns the unit count and shows where to draw on a U-100 insulin syringe. If you are working from a vial that is not in our catalog, the standalone peptide reconstitution calculator takes any vial strength and volume and prints its working.
Educational reference, not a prescription and not a personal dose. This tool converts a concentration into syringe units so you can check the math. It does not set a dose or tell you how to inject. A licensed clinician sets any dose and administration.
Equivalent on a U-100 syringe
50units
At this concentration, 5 mg corresponds to about 50 units on a standard U-100 insulin syringe. This is a math reference, not a dose or an instruction.
Recommended bac water volumes
Default reconstitution presets for every Peptara Labs SKU. The example amounts are round figures picked to make the unit arithmetic easy to follow, not a recommendation for any vial, any compound or any person. A licensed clinician sets any dose.
Peptide reconstitution chart
Read this peptide reconstitution chart as a math illustration only: each row shows how a vial size and a 2mL bac water fill convert an example amount into syringe units. It is not a dose for anyone, and nothing here picks one.
| Peptide | Vial size | Bac water | Example amount (not a recommendation) | Units per example amount |
|---|---|---|---|---|
| Retatrutide | 20mg | 2mL | 5mg | 50 units |
| Tirzepatide | 20mg | 2mL | 5mg | 50 units |
| BPC-157 | 20mg | 2mL | 250mcg | 3 units |
| CJC-1295 / Ipamorelin | 10mg | 2mL | 250mcg | 5 units |
| GHK-Cu | 100mg | 2mL | 2mg | 4 units, draw slowly: at 50mg/mL a single unit is 0.5mg |
| TB-500 | 10mg | 2mL | 2.5mg | 50 units |
| MT-2 (Melanotan-2) | 10mg | 2mL | 0.5mg | 10 units |
| Semax | 10mg | 2mL | 600mcg | 12 units |
| Selank | 10mg | 2mL | 600mcg | 12 units |
| KLOW Combo | 80mg | 2mL | 4mg | 10 units |
| MOTS-c | 40mg | 2mL | 5mg | 25 units (matches JP protocol Section 06) |
| Epithalon | 50mg | 2mL | 1mg | 4 units, draw slowly: at 25mg/mL a single unit is 0.25mg |
| Tesamorelin | 20mg | 2mL | 2mg | 20 units |
| SLU-PP-332 | 5mg | 2mL | 1mg | 40 units |
| PT-141 (Bremelanotide) | 10mg | 2mL | 1.5mg | 30 units |
| Glutathione | 600mg | 2mL | 200mg | 67 units at 300mg/mL, drawn across one syringe |
Figures shown for math illustration only. They are not a dose for anyone, and nothing on this page picks one.
Storage
Before reconstitution
Stable at room temperature
Lyophilized peptide powder is stable at room temperature for weeks and in a freezer for many months. Keep the vial sealed and out of direct sunlight until the day you reconstitute.
After reconstitution
Refrigerate immediately
Once mixed with bac water, store the vial at 2 to 8 Celsius. 30-day shelf life is the safe default for most peptides. The per-compound pages carry the per-peptide detail.
Travel
Reconstituted vials travel with an ice pack in carry-on. Lyophilized vials handle cabin temperature without ice. Country-specific customs rules vary, see the FAQ for travel guidance.
Frequently asked questions
Peptide pages
Retatrutide
Triple-agonist GLP-1 / GIP / glucagon. Studied in clinical trials for weight loss.
View page
Tirzepatide
Dual-agonist GLP-1 / GIP. Studied in clinical trials for weight loss.
View page
BPC-157
Body Protection Compound for tissue repair, gut healing, recovery.
View page
Compound-specific guides
How to reconstitute retatrutide
The 20mg vial start to finish, what bac water is, how much to add, and the units math worked out at 1 mL, 2 mL and 3 mL.
Read the guide
Order with reconstitution math built in
The concentration math for every vial we sell, worked out on this page, in units rather than mL.
Every Peptara Labs order includes bac water sized to the vial, a printed reference, and support on WhatsApp for the vial, the volume and storage.